CST1, 21-141aa, Human

Proteins, Recombinant
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Product Name CST1, 21-141aa, Human
Applications SDS-PAGE
Background CST1 (Cystatin-SN) is a member of the type 2 salivary cystatin family found in a variety of fluids and secretions, including plasma, tears, and saliva. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. CST1 was upregulated in cancerous lesions of gastric cancer tissues compared to noncancerous regions and clinicopathological analysis showed a significant correlation between high expression of CST1. Recombinant human CST1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Concentration 0.25 mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
Species Human
Host E.coli
Intended Use For Research Use Only
Molecular Weight 30.3 kDa (277aa), confirmed by MALDI-TOF
NCBI Accession # NP_001889
Purity > 95% by SDS-PAGE
References Choi EH, et al. (2009) Clin Chim Acta. 406(1-2):45-51.
Ryan C.M., et al. (2010) J. Am. Soc. Mass Spectrom. 21:908-917
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Synonyms cystatin SN, Cystain-SA-I, Cystatin-1, Cystatin-SN, Salivary cystatin-SA-1
Tag His-tag

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