DCTD, 1-178aa, Human

Proteins, Recombinant
More Information
Product Name DCTD, 1-178aa, Human
Background DCTD (deoxycytidylate deaminase), also known as dCMP deaminase, is a 178 amino acid allosteric enzyme that exists as a homohexamer and belongs to the cytidine and deoxycytidylate deaminase protein family. Using zinc as a cofactor, DCTD catalyzes the deamination of dCMP to dUMP, thereby producing the nucleotide substrate (dUMP) that is used by thymidylate synthase. Recombinant human DCTD protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques
Species Human
Synonyms Deoxycytidylate deaminase, dCMP deaminase, MGC111062
Host E.coli
Tag His-tag
Molecular Weight 42.2 kDa (378aa) confirmed by MALDI-TOF
Form Liquid, in 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol
Concentration 1 mg/ml (determined by Bradford assay)
Purity > 90% by SDS-PAGE
Applications SDS-PAGE
NCBI NP_001912
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Okazaki T., et al. (2009) Clin Cancer Res. 16(1):320-9.
Pazy Y., et al. (2009) J Mol Biol. 392(5):1205-20.

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