DDT, 1-118aa, Human

Proteins, Recombinant
More Information
Product Name DDT, 1-118aa, Human
Background DDT is an enzyme that catayzes the tautomerization of D-dopachrome to give 5,6-dihydroxyindole (DHI). This protein belongs to the family of lyases, specifically the carboxy-lyases, which cleave carbon-carbon bonds. It shares a homologous amino acid sequence (33% identical) with the macrophage migration inhibitory factor (MIF) and possesses similar tautomerase activity. In addition, it may functions a proinflammatory cytokine. Recombinant human DDT, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Species Human
Synonyms DDCT, D-dopachtome decarboxylase, D-dopachrome tautomerase, D dopachrome decarboxylase, D dopachrome tautomerase, Phenylpyruvate tautomerase II,
Host E.coli
Molecular Weight 13.7 kDa (119aa), confirmed by MALDI-TOF
Form Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 1mM EDTA, 50mM NaCl
Concentration 1mg/ml (determined by Bradford assay)
Purity > 95% by SDS-PAGE
Applications SDS-PAGE
NCBI NP_001346
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Arisawa K., et al. (2009) Toxicology. 255(1-2):6-14.
Nishihira J., et al. (1999) Biochemistry. 38(11):3268-79.

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