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|Product Name||DUT, 70-252aa, Human|
|Amino Acid Sequence||MGSSHHHHHH SSGLVPRGSH MASTVGAAGW KGELPKAGGS PAPGPETPAI SPSKRARPAE VGGMQLRFAR LSEHATAPTR GSARAAGYDL YSAYDYTIPP MEKAVVKTDI QIALPSGCYG RVAPRSGLAA KHFIDVGAGV IDEDYRGNVG VVLFNFGKEK FEVKKGDRIA QLICERIFYP EIEEVQALDD TERGSGGFGS TGKN|
|Background||DUT, also known as dUTP pyrophosphatase, is a ubiquitous enzyme that functions in nucleotide metabolism. This protein, in the presence of magnesium ions, is responsible for hydrolyzing dUTP to dUMP and diphosphate. This reaction is important for keeping the intracellular dUTP concentration low so that uracil does not become incorporated into DNA. Extensive incorporation of uracil into DNA can ultimately lead to cell death. This suggests that DUT is essential for cell viability, further implying that it is a potential target for anticancer therapy. Recombinant human DUT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
|Concentration||0.5 mg/ml (determined by Bradford assay)|
|Form||Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 0.1M NaCl,1mM DTT|
|Intended Use||For Research Use Only|
|Molecular Weight||26.8 kDa (250aa), confirmed by MALDI-TOF|
|NCBI Accession #||NP_001020419|
|Purity||> 90 % by SDS-PAGE|
|References||Mclntosh EM., et al. (1992) Proc Natl Acad Sci U S A. 89(17):8020-4.
Persson R., et al. (2001) Curr Protein Pept Sci. 2(4):287-300.
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Synonyms||dUTPase, dUTP pyrophosphatase, FLJ20622, Deoxyuridine 5-triphosphate nucleotidohydrolase mitochondrial, Deoxyuridine Triphosphatase|
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