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|Product Name||ECHDC1, 1-307aa, Human|
|Amino Acid Sequence||MGSSHHHHHH SSGLVPRGSH MALKQEMAKS LLKTASLSGR TKLLHQTGLS LYSTSHGFYE EEVKKTLQQF PGGSIDLQKE DNGIGILTLN NPSRMNAFSG VMMLQLLEKV IELENWTEGK GLIVRGAKNT FSSGSDLNAV KSLGTPEDGM AVCMFMQNTL TRFMRLPLIS VALVQGWALG GGAEFTTACD FRLMTPESKI RFVHKEMGII PSWGGTTRLV EIIGSRQALK VLSGALKLDS KNALNIGMVE EVLQSSDETK SLEEAQEWLK QFIQGPPEVI RALKKSVCSG RELYLEEALQ NERDLLGTVW GGPANLEAIA KKGKFNK|
|Background||ECHDC1, also known as enoyl CoA hydratase domain containing 1, belongs to the enoyl-CoA hydratase/isomerase family. Enoyl-CoA hydratase is an enzyme that hydrates the double bond between the second and third carbons on acyl-CoA. This enzyme is essential to metabolizing fatty acids to produce both acetyl CoA and energy. Recombinant human ECHDC1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.|
|Concentration||0.5mg/ml (determined by Bradford assay)|
|Form||Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 150mM NaCl|
|Intended Use||For Research Use Only|
|Molecular Weight||85.6kDa (743aa)|
|NCBI Accession #||NP_001132982|
|Purity||> 95% by SDS-PAGE|
|References||Bahnson. et al. (2007) Biochemistry 41: 2621-2629.
Menachem TD. et al. (2009) Fam Cancer. 8:399-402.
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Synonyms||Enoyl CoA hydratase domain containing 1, dJ351K20.2|
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