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|Product Name||GCSH, 49-173aa, Human|
|Amino Acid Sequence||MGSSHHHHHH SSGLVPRGSH MGSMSVRKFT EKHEWVTTEN GIGTVGISNF AQEALGDVVY CSLPEVGTKL NKQDEFGALE SVKAASELYS PLSGEVTEIN EALAENPGLV NKSCYEDGWL IKMTLSNPSE LDELMSEEAY EKYIKSIEE|
|Background||The enzyme system for cleavage of glycine (glycine cleavage system) is composed of four mitochondrial protein components: P protein, H protein, T protein, and L protein. GCSH is the H protein, which transfers the methylamine group of glycine from the P protein to the T protein. Defects in this gene are a cause of nonketotic hyperglycinemia (NKH). Recombinant human GCSH protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
|Concentration||1 mg/ml (determined by Bradford assay)|
|Form||Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 0.4M Urea, 10% glycerol|
|Intended Use||For Research Use Only|
|Molecular Weight||25.3 kDa (229aa)|
|NCBI Accession #||NP_004474|
|Purity||> 95 % by SDS-PAGE|
|References||Kure S., et al. (2001) J Hum Genet. 46(7):378-84.
Kovata H., et al. (1991) Am J Hum Genet. 48(2):351-61.
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Synonyms||Glycine cleavage system protein H (aminomethyl carrier), GCE, NKH|
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