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|Product Name||GDF-5, 382-501aa, Human, Recombinant|
|Amino Acid Sequence||MGSSHHHHHH SSGLVPRGSH MAPLATRQGK RPSKNLKARC SRKALHVNFK DMGWDDWIIA PLEYEAFHCE GLCEFPLRSH LEPTNHAVIQ TLMNSMDPES TPPTCCVPTR LSPISILFID SANNVVYKQY EDMVVESCGC R|
|Background||Growth differentiation factor 5(GDF-5) is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This protein plays a role in chondrogenesis and chondrocyte metabolism, tendon and ligament tissue formation, and bone repair. It also increases the survival of neurones that respond to a neurotransmitter called dopamine, and is a potential therapeutic molecule associated with Parkinson's disease. GDF-5, fused to His-tag at N-terminus, was expressed as insoluble protein aggregate in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.|
|Concentration||1 mg/ml (determined by Bradford assay)|
|Endotoxin Level||< 1.0 EU per 1 microgram of protein (determined by LAL method)|
|Form||Liquid, in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol|
|Intended Use||For Research Use Only|
|Molecular Weight||36.4 kDa (305aa), confirmed by MALDI-TOF.|
|NCBI Accession #||NP_000548|
|Purity||> 95% by SDS-PAGE|
|References||Feng G., et al. (2008). Growth Factor. 26(3):132-42
Sullivan AM., et al. (2005). J Anat. 207(3):219-26.
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Synonyms||Growth differentiation factor 5, BMP14, CDMP1, LAP4, OS5, SYNS2, Growth differentiation factor 5 Cartilage derived morphogenetic protein 1,|
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