| Product Name | GOLGA7, 1-137aa, Human His tag |
|---|---|
| Description | GOLGA7 belongs to the ERF4 family. This protein may be involved in protein transport from Golgi to cell surface. The ZDHHC9-GOLGA7 complex is a palmitoyltransferase specific for HRAS and NRAS. GOLGA7 is palmitoylated on Cys-69 and Cys-72; which is required for Golgi localization and interaction with GOLGA3. Recombinant human GOLGA7 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | Golgin subfamily A member 7 isoform a, GCP16, GOLGA3AP1, GOLGA7A, HSPC041 |
| Host | E.coli |
| Molecular Weight | 21.2 kDa (187aa) confirmed by MALDI-TOF |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSMRPQQAP VSGKVFIQRD YSSGTRCQFQ TKFPAELENR IDRQQFEETV RTLNNLYAEA EKLGGQSYLE GCLACLTAYT IFLCMETHYE KVLKKVSKYI QEQNEKIYAP QGLLLTDPIE RGLRVIEITI YEDRGMSSGR |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 1mM DTT, 1mM EDTA, 250mM imidazole |
| Concentration | 0.25 mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
| References | Ohta E, Misumi Y, et al. (2003). J. Biol. Chem. 278:51957-51967. |
| Supplier | ARP |
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