Product Name | GRHPR, 1-328aa, Human |
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Description | GRHPR, also known as GLXR, is a member of the D-isomer specific 2-hydroxyacid dehydrogenase family of proteins. Localizing to the cytosol, GRHPR is ubiquitously expressed with highest expression levels found in liver. The protein has widespread tissue expression and has a role in metabolism. GRHPR is an enzyme with hydroxypyruvate reductase, glyoxylate reductase, and D-glycerate dehydrogenase enzymatic activities. Recombinant human GRHPR protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques |
Synonyms | GLXR, GLYD, PH2, Glyoxylate reductase/hydroxypyruvate reductase glycerate 2 dehydrogenase, Glyoxylate reductase/hydroxypyruvate reductase, OTTHUMP00000046131, PH 2, Primary hyperoxaluria type 2, |
Host | E.coli |
Molecular Weight | 37.6 kDa (352aa), confirmed by MALDI-TOF |
Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MRPVRLMKVF VTRRIPAEGR VALARAADCE VEQWDSDEPI PAKELERGVA GAHGLLCLLS DHVDKRILDA AGANLKVIST MSVGIDHLAL DEIKKRGIRV GYTPDVLTDT TAELAVSLLL TTCRRLPEAI EEVKNGGWTS WKPLWLCGYG LTQSTVGIIG LGRIGQAIAR RLKPFGVQRF LYTGRQPRPE EAAEFQAEFV STPELAAQSD FIVVACSLTP ATEGLCNKDF FQKMKETAVF INISRGDVVN QDDLYQALAS GKIAAAGLDV TSPEPLPTNH PLLTLKNCVI LPHIGSATHR TRNTMSLLAA NNLLAGLRGE PMPSELKL |
Tag | His-tag |
Reactivity | Human |
Applications | SDS-PAGE |
Form | Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 0.1M NaCl |
Concentration | 1 mg/ml (determined by Bradford assay) |
Purity | > 95% by SDS-PAGE |
Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
References |
Booth MP., et al. (2006) J Mol Biol. 360(1):178-89. Genolet R., et al. (2005) J Biol Chem. 280(25):24143-52. |
Supplier | ARP |
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