| Product Name | H3N2/HA, 18-344aa, H3N2/HA (Canine), Baculovirus |
|---|---|
| Description | Influenza A virus subtype H3N2, also known as H3N2, is a subtype of viruses that cause influenza (flu). H3N2 Viruses can infect birds and mammals. In birds, humans, and pigs, the virus has mutated into many strains. Hemagglutinin(HA) binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. It plays a major role in the determination of host range restriction and virulence and is responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Recombinant Influenza A virus (H3N2, canine) HA protein, fused to His-tag at C-terminus, was expressed in Hi-5 cell and purified by using conventional chromatography techniques. |
| Synonyms | Hemagglutinin |
| Host | E.coli |
| Molecular Weight | 35.8kDa (315aa) |
| Amino Acid Sequence | ADNLPGNENN AATLCLGHHA VPNGTIVKTI TDDQIEVTNA TELVQNSSTG KICNNPHKIL DGRDCTLIDA LLGDPHCDVF QNETWDLFVE RSNAFSNCYP YDVPDYASLR SIVASSGTLE FITEGFTWAG VTQNGGSGAC KKGPANGFFS RLNWLTKSGN TYPVLNVTMP NNNNFDKLYI WGVHHPSTNQ EQTSLYIQAS GRVKVSTRRS QQTIIPNIGS RPLVRGQSGR ISVYWTIVKP GDVLVINSNG NLIAPRGYFK MRIGKSSIMR SDAPIDTCIS ECITPNGSIP NEKPFQNVNK ITYGACPKYV KQNTLKLATG MRNVPERQTH HHHHH |
| Tag | His-tag |
| Reactivity | Virus |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 100mM NaCl, 0.1mM PMSF |
| Concentration | 0.25mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
| References |
Li S., et al. (2010) Infect. Genet. Evol. 10:1286-1288 Song D., et al. (2008) Emerg. Infect. Dis. 14:741-746 |
| Supplier | ARP |
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