| Product Name | HCV NS3, 1225-1456aa, HCV |
|---|---|
| Description | HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. Nonstructural (NS) proteins of HCV play major roles in viral replication and the pathogenesis of liver diseases. The NS3 protein of hepatitis C virus (HCV) contains protease and RNA helicase activities, both of which are likely to be essential for HCV propagation. Recombinant HCV NS3 protein was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | , NS3 protease/helicase HCV NS3 transactivated protein, NS 3, NS3P, P70, Serine protease/NTPase/helicase. |
| Host | E.coli |
| Molecular Weight | 23.1kDa (208aa), confirmed by MALDI-TOF. |
| Amino Acid Sequence | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSVAHL HAPTGSGKST KVPAAYAAQG YKVLVLNPSV AATLGFGAYM SKAHGVDPNI RTGVRTITTG SPITYSTYGK FLADGGCSGG AYDIIICDEC HSTDATSILG IGTVLDQAET AGARLVVLAT ATPPGSVTVS HPNIEEVALS TTGEIPFYGK AIPLEVIKGG RHLIFCHSKK KCDELAAKLV ALGINAVAYY RGLDVSVIPT SGDVVVVSTD ALMTGFTGDF DSVIDCNT |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 200mM NaCl, 2mM DTT, 40% glycerol |
| Concentration | 0.25 mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
| References |
Hahn JA., et al. (2007) J Infect Dis. 195(11):1556-9. Caruntu FA., et al. (2006) J Gastrointestin Live Dis. 15(3):249-56. |
| Supplier | ARP |
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