Hexokinase 4 Human, Recombinant

Category: Proteins
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01-2274
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Product Name Hexokinase 4 Human, Recombinant
Description Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase4 is found in the liver and pancreatic beta-cells, where it is controlled by insulin (activation) and glucagon (inhibition). In pancreatic beta-cells, type IV enzyme acts as a glucose sensor to modify insulin secretion. Hexokinase4 is commonly called ‘glucokinase'. Recombinant human Hexokinase4, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms GCK, Hexokinase 4, EC 2.7.1.2, Hexokinase type IV, HK IV, Hexokinase-4, HK4, Hexokinase-D, Glucokinase, ATP D hexose 6 phosphotransferase, GK, GLK, Glucokinase, Glucokinase isoform 1, Hexokinase 4, Hexokinase D, Hexokinase D pancreatic isozyme, Hexokinase4, HexokinaseD, HHF 3, HHF3, HK 4, HXKP, Maturity onset diabetes of the young 2, MODY 2, MODY2.
Host E.coli
Molecular Weight 100.9kDa (894aa)
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MLDDRARMEA AKKEKVEQIL AEFQLQEEDL KKVMRRMQKE MDRGLRLETH EEASVKMLPT YVRSTPEGSE VGDFLSLDLG GTNFRVMLVK VGEGEEGQWS VKTKHQMYSI PEDAMTGTAE MLFDYISECI SDFLDKHQMK HKKLPLGFTF SFPVRHEDID KGILLNWTKG FKASGAEGNN VVGLLRDAIK RRGDFEMDVV AMVNDTVATM ISCYYEDHQC EVGMIVGTGC NACYMEEMQN VELVEGDEGR MCVNTEWGAF GDSGELDEFL LEYDRLVDES SANPGQQLYE KLIGGKYMGE LVRLVLLRLV DENLLFHGEA SEQLRTRGAF ETRFVSQVES DTGDRKQIYN ILSTLGLRPS TTDCDIVRRA CESVSTRAAH MCSAGLAGVI NRMRESRSED VMRITVGVDG SVYKLHPSFK ERFHASVRRL TPSCEITFIE SEEGSGRGAA LVSAVACKKA CMLGQ
Tag His-tag
Reactivity Human
Applications SDS-PAGE
Form Liquid, in 20 mM Tris pH 8.0, 50 mM NaCl
Concentration 1 mg/ml (determined by Bradford assay)
Purity > 95% by SDS-PAGE
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
References Jon E. et al.,(2003) J.Exp Biology. 206: 2049-2057
Supplier ARP

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