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|Product Name||HLA-DOB, 27-224aa, Human|
|Amino Acid Sequence||MGSSHHHHHH SSGLVPRGSH MGSGTDSPED FVIQAKADCY FTNGTEKVQF VVRFIFNLEE YVRFDSDVGM FVALTKLGQP DAEQWNSRLD
LLERSRQAVD GVCRHNYRLG APFTVGRKVQ PEVTVYPERT PLLHQHNLLH CSVTGFYPGD IKIKWFLNGQ EERAGVMSTG PIRNGDWTFQ
TVVMLEMTPE LGHVYTCLVD HSSLLSPVSV EWRAQSEYSW RK
|Background||HLA-DOB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DOA) and a beta chain (DOB), both anchored in the membrane. It is located in intracellular vesicles. DO suppresses peptide loading of MHC class II molecules by inhibiting HLA-DM. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Recombinant human HLA-DOB protein, fused to His-tag at N-terminus, was expressed in E.coli.|
|Concentration||1mg/ml (determined by Bradford assay)|
|Form||Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 30% glycerol 0.15M NaCl, 1mM DTT|
|Intended Use||For Research Use Only|
|Molecular Weight||.6 kDa (161aa) confirmed by MALDI-TOF|
|NCBI Accession #||NP_002111|
|Purity||> 95% by SDS-PAGE|
|References||Naruse T.K., et al. (2002) Tissue Antigens. 59:512-519
Beck S., et al. (1996) J. Mol. Biol. 255:1-13
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Synonyms||Major histocompatibility complex, class II, DO beta, DOB|
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