Human Sphingosine kinase 2 (SPHK2) ELISA Kit

Catalog
KTE60528
$0.00
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Assay Kits, ELISA, Human ELISA, Abbkine
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Product Name Human Sphingosine kinase 2 (SPHK2) ELISA Kit
Description This Human Sphingosine kinase 2 (SPHK2) ELISA Kit employs a two-site sandwich ELISA to quantitate SPHK2 in samples. An antibody specific for SPHK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPHK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPHK2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPHK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background Sphingosine kinase 2 (SPHK2) is one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. SPHK2 may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for SPHK2.
Method Sandwich ELISA
Gene ID 56848
Species Human
Intended Use For Research Use Only
Sample Types Cell culture supernatants, Serum, Plasma, Other biological fluids
Synonyms SPHK2, Sphingosine kinase type 2 isoform
Uniprot Accession ID Q9NRA0

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