| Product Name | IGF-1, Human, Recombinant |
|---|---|
| Description | The Insulin-like growth factor-1(IGF-1) is mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. IGF-1 is predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGF-1 belongs to the insulin gene family, which also contains insulin and relaxin. The IGF-1 is similar by structure and function to insulin, but have a much higher growth-promoting activity than insulin. This recombinant human IGF-I is globular protein containing 70 amino acids, and 3 intra-molecular disulfide bonds. Recombinant IGF-1 was expressed in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. |
| Synonyms | Insulin-like growth factor-1, IGF-IA, Somatomedin-C, MGF, IBP1, IGF1, IBP1, IGF-1, Insulin-like growth factor-1 IGF IA, IGF IB, IGF1, IGFI, IGFIA, Insulin like growth factor 1, Insulin like growth factor 1 (somatomedin C), Insulin like growth factor IA, Insulin like growth factor IB, Mechano growth factor, Somatomedin C, |
| Host | E.coli |
| Molecular Weight | 104.1kDa (937aa) |
| Amino Acid Sequence | MGPETLCGAE LVDALQFVCG DRGFYFNKPT GYGSSSRRAP QTGIVDECCF RSCDLRRLEM YCAPLKPAKS A |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl pH8.0, 10% glycerol |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
| References |
Rinderknecht E., et al (1978) J Biol.Chem, 253: 2769. Zumstein P., et al (1987) J Biol. Chem, 262: 11252. Rabinovsky ED (2004) Neurol Res, 26: 204-210 |
| Supplier | ARP |
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