KIR2DL1 (p58 KIR, CD158), Human, Recombinant, Recombinant

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01-2210
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Proteins, Recombinant
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Product Name KIR2DL1 (p58 KIR, CD158), Human, Recombinant, Recombinant
Amino Acid Sequence MEGVHRKPSL LAHPGRLVKS EETVILQCWS DVMFEHFLLH REGMFNDTLR LIGEHHDGVS KANFSISRMT QDLAGTYRCY GSVTHSPYQV SAPSDPLDIV IIGLYEKPSL SAQLGPTVLA GENVTLSCSS RSSYDMYHLS REGEAHERRL PAGPKVNGTF QADFPLGPAT HGGTYRCFGS FHDSPYEWSK SSDPLLVSVT GN
Applications SDS-PAGE
Background An inhibitory Keller Cell Ig-like Receptor(KIR, previously called p58 KIR, p58.1, cl-42, NKAT1, or KIR-K6), which recognizes class I MHC molecules(HLA-Cw2, -Cw4, -Cw5, and Cw6). The protein coding region of the extracellular domain of KIR2DL1(amino acids 1-202) was cloned into an E. coli expression vector. The extracellular domain of KIR2DL1 was overexpressed as insoluble protein aggregates(inclusion bodies). The recombinant KIR2DL1 protein was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer.
Concentration 1 mg/ml (determined by Bradford assay)
Form Liquid, in 50mM Tris pH 8.0, 0.1mM PMSF, 1mM EDTA, 1mM DTT, 10% glycerol.
Species Human
Host E.coli
Intended Use For Research Use Only
Molecular Weight 20kDa (179aa), confirmed by MALDI-TOF.
NCBI Accession # NP_055033
Purity > 95% by SDS-PAGE
References Steffens, U. et al. (1998) Tissue Antigens 51, 398-413.
Wagtmann, N.R., et al. (1995) Immunity 2, 439-449.
Colonna, M. et al. (1995) Science 269, 405-408;
Kim, J. et al. (1997) J. Immunol. 159, 3875-3882.
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Synonyms KIR2DL1, CD158A, NKAT1, KIR2DL1 (p58 KIR, CD158), MHC class I NK cell receptor, Natural killer-associated transcript 1, NKAT-1, p58 natural killer cell receptor clones CL-42/47.11, p58 NK receptor, p58.1 MHC class-I-specific NK receptor, Killer cell immunoglobulin-like receptor 2DL1, Killer cell immunoglobulin-like receptor 2DL1 Killer cell immunoglobulin like receptor 2DL1, MHC class I NK cell receptor, Natural killer associated transcript 1, NKAT 1, p58 NK receptor, p58.1 MHC class I specific NK receptor
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