MCFD2, 27-146aa, Human, T7 tag

Proteins, Recombinant
More Information
Product Name MCFD2, 27-146aa, Human, T7 tag
Background Multiple coagulation factor deficiency 2 (MCFD2), also known as SDNSF. This is expressed by neural stem/progenitor cells of the hippocampus, and localized to region where neurogenesis persists throughout life. It has been found to prevent NSC cell death and to maintain stem cell characteristics. This protein forms a complex with LAMN1 that facilitates the transport of coagulation factors V and VIII from the endoplasmic reticulum to the Golgi apparatus via an endoplasmic reticulum Golgi intermediate compartment. Mutations in the MCFD2 gene may cause Factor V and Factor VIII combined deficiency. Recombinant human MCFD2, fused to T7-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Species Human
Synonyms SDNSF, LMAN1IP, Multiple coagulation factor deficiency 2 MCFD 2, DKFZp686G21263, F5F8D, Neural stem cell derived neuronal survival protein, Multiple coagulation factor deficiency protein 2.
Host E.coli
Molecular Weight 47.9 kDa (427aa), confirmed by MALDI-TOF.
Form Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1 M NaCl.
Concentration 1 mg/ml (determined by Bradford assay)
Purity > 90% by SDS-PAGE
Applications SDS-PAGE
NCBI NP_644808
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Intended Use For Research Use Only
References Zhanq B., et al. (2005) Biol Chem. 280(27):25881-6.
Mohanty D., et al. (2005) Am J Hematol. 79:262-266.

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