Mouse Pituitary tumor-transforming gene 1 protein-interacting protein (PTTG1IP) ELISA Kit
| Product Name | Mouse Pituitary tumor-transforming gene 1 protein-interacting protein (PTTG1IP) ELISA Kit |
|---|---|
| Description | Mouse Pituitary tumor-transforming gene 1 protein-interacting protein (PTTG1IP) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PTTG1IP. No significant cross-reactivity or interference between Mouse PTTG1IP and analogues was observed. PTTG1IP, which directly binds to pituitary tumor-transforming gene 1 protein (PTTG1), facilitates the nuclear translocation of PTTG1 and potentiates the transcriptional activation of basic fibroblast growth factor by PTTG1. The gene product localizes to both the cytoplasm and nucleus. Its NLS is required for its own nuclear localization, the nuclear localization of PTTG1, and its interaction with PTTG1. The predicted 180-amino acid C21ORF3 protein has features of a type Ia integral membrane protein and contains the tetrapeptide YXRF, a motif observed in proteins internalized via coated pit-mediated endocytosis. Northern blot analysis detected a 2.69-kb C21ORF3 mRNA in all tissues examined. This Mouse Pituitary tumor-transforming gene 1 protein-interacting protein (PTTG1IP) ELISA Kit employs a two-site sandwich ELISA to quantitate PTTG1IP in samples. An antibody specific for PTTG1IP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTTG1IP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTTG1IP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTTG1IP bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | PTTG1IP, C21orf1, C21orf3, PBF, PTTG-binding factor, pituitary tumor-transforming gene 1 protein-interacting protein, pituitary tumor-transforming gene protein-binding factor |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Mouse |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 108705 |
| Storage | 2-8°C |
| Supplier | Abbkine |
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