Mouse Ras-related protein M-Ras (MRAS) ELISA Kit

Category: Assay Kits
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KTE70956
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Product Name Mouse Ras-related protein M-Ras (MRAS) ELISA Kit
Description Mouse Ras-related protein M-Ras (MRAS) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MRAS. No significant cross-reactivity or interference between Mouse MRAS and analogues was observed. MRAS encodes a member of the Ras family of small GTPases. These membrane-associated proteins function as signal transducers in multiple processes including cell growth and differentiation, and dysregulation of Ras signaling has been associated with many types of cancer. The encoded protein may play a role in the tumor necrosis factor-alpha and MAP kinase signaling pathways. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. This Mouse Ras-related protein M-Ras (MRAS) ELISA Kit employs a two-site sandwich ELISA to quantitate MRAS in samples. An antibody specific for MRAS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMRAS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MRAS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MRAS bound in the initial step. The color development is stopped and the intensity of the color is measured.
Synonyms MRAS, FLJ42964, M-RAs, R-RAS3, RRAS3, muscle and microspikes RAS
Method Sandwich ELISA
Detection Range Please inquire
Sensitivity Please inquire
Reactivity Mouse
Sample Types Cell culture supernatants, Serum, Plasma, Other biological fluids
Gene Id 17532
Storage 2-8°C
Supplier Abbkine

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