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|Product Name||MVD, 1-400aa, Human|
|Amino Acid Sequence||MGSSHHHHHH SSGLVPRGSH MASEKPLAAV TCTAPVNIAV IKYWGKRDEE LVLPINSSLS VTLHQDQLKT TTTAVISKDF TEDRIWLNGR EEDVGQPRLQ ACLREIRCLA RKRRNSRDGD PLPSSLSCKV HVASVNNFPT AAGLASSAAG YACLAYTLAR VYGVESDLSE VARRGSGSAC RSLYGGFVEW QMGEQADGKD SIARQVAPES HWPELRVLIL VVSAEKKLTG STVGMRASVE TSPLLRFRAE SVVPARMAEM ARCIRERDFP SFAQLTMKDS NQFHATCLDT FPPISYLNAI SWRIIHLVHR FNAHHGDTKV AYTFDAGPNA VIFTLDDTVA EFVAAVWHGF PPGSNGDTFL KGLQVRPAPL SAELQAALAM EPTPGGVKYI IVTQVGPGPQ ILDDPCAHLL GPDGLPKPAA|
|Background||MVD, also known as diphosphomevalonate decarboxylase, catalyzes the conversion of mevalonate pyrophosphate into isopentenyl pyrophosphate in one of the early steps in cholesterol biosynthesis. It decarboxylates and dehydrates its substrate while hydrolyzing ATP. Recombinant human MVD protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.|
|Concentration||0.5 mg/ml (determined by Bradford assay)|
|Form||Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 1mM DTT, 0.1M NaCl|
|Intended Use||For Research Use Only|
|Molecular Weight||46.9 kDa (437aa), confirmed by MALDI-TOF|
|NCBI Accession #||NP_002452|
|Purity||> 85% by SDS-PAGE|
|References||Toth M.J., et al. (1996) J. Biol. Chem. 271:7895-7898
Molina H., et al. (2007) Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Synonyms||diphosphomevalonate decarboxylase, MPD|
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