NDE1, 1-335aa, Human

Category: Proteins
Catalog
01-P1027
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Product Name NDE1, 1-335aa, Human
Description NDE1 is a cytoplasmic protein belonging to the nudE protein family. Phosphorylated during mitosis, it is essential for the formation and function of the mitotic spindle in M phase and functions to regulate the Dynein-mediated transport of kinetochore proteins, as well as centrosome duplication during interphase. NDE1 is thought to interact with NDEL1, LIS1 and Dynein IC1/2, cytosolic in a signaling pathway that regulates the formation of neurons and is fundamental to the development of the cerebral cortex. Recombinant human NDE1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms Nuclear distribution protein nudE homolog 1, FLJ20101, HOM-TES-87, NUDE, NUDE, nudE nuclear distribution gene E homolog 1 (A. nidulans)
Host E.coli
Molecular Weight 21.9 kDa (194aa) confirmed by MALDI-TOF
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MEDSGKTFSS EEEEANYWKD LAMTYKQRAE NTQEELREFQ EGSREYEAEL ETQLQQIETR NRDLLSENNR LRMELETIKE KFEVQHSEGY RQISALEDDL AQTKAIKDQL QKYIRELEQA NDDLERAKRA TIMSLEDFEQ RLNQAIERNA FLESELDEKE NLLESVQRLK DEARDLRQEL AVQQKQEKPR TPMPSSVEAE RTDTAVQATG SVPSTPIAHR GPSSSLNTPG SFRRGLDDST GGTPLTPAAR ISALNIVGDL LRKVGALESK LASCRNLVYD QSPNRTGGPA SGRSSKNRDG GERRPSSTSV PLGDKGLDTS CRWLSKSTTR SSSSC
Tag His-tag
Reactivity Human
Applications SDS-PAGE
Form Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT
Concentration 1.0 mg/ml (determined by Bradford assay)
Purity > 85 % by SDS-PAGE
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
References Yan X., et al. (2003) Mol Cell Biol. 23(4):1239-50.
Kitagawa M., et al. (2000) FEBS Lett. 479(1-2):57-62.
Supplier ARP

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