Otubain 1, 1-271aa, Human, Recombinant

Category: Proteins
Catalog
01-2138
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Product Name Otubain 1, 1-271aa, Human, Recombinant
Description Otubain 1, also known as OTUB1, is a member of the OUT (ovarian tumor) superfamily of predicted cysteine proteases and inhibits cytokine gene transcription in the immune system via its interaction with a ubiquitin protease and E3 ubiquitin ligase. This protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function of polyubiquitin chain growth. Recombinant OTUB1 protein was expressed in E.coli and purified by using conventional chromatography techniques.
Synonyms OTB1, OTU1, Ubiquitin thioesterase Otubain 1 Deubiquitinating enzyme OTUB1, OTU domain containing ubiquitin aldehyde binding protein 1, Otubain 1, Ubiquitin specific processing protease OTUB1, Ubiquitin thioesterase OTUB1.
Host E.coli
Molecular Weight 41.2 kDa (365aa), confirmed by MALDI-TOF.
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MAAEEPQQQK QEPLGSDSEG VNCLAYDEAI MAQQDRIQQE IAVQNPLVSE RLELSVLYKE YAEDDNIYQQ KIKDLHKKYS YIRKTRPDGN CFYRAFGFSH LEALLDDSKE LQRFKAVSAK SKEDLVSQGF TEFTIEDFHN TFMDLIEQVE KQTSVADLLA SFNDQSTSDY LVVYLRLLTS GYLQRESKFF EHFIEGGRTV KEFCQQEVEP MCKESDHIHI IALAQALSVS IQVEYMDRGE GGTTNPHIFP EGSEPKVYLL YRPGHYDILY K
Tag His-tag
Reactivity Human
Applications SDS-PAGE
Form Liquid, in 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
Concentration 1 mg/ml (determined by Bradford assay)
Purity > 95% by SDS-PAGE
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
References Balakirev MY., et al. (2003) EMBO Rep. 4(5):517-22.
Soares L., et al. (2004) Nat Immunol. 5(1):45-54.
Supplier ARP

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