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|Product Name||PCMT, 1-227aa, Human|
|Background||PCMT1, also known as L-isoaspartyl protein carboxyl methyltransferase, is an enzyme that catalyses the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. This protein plays a role in the repair and/or degradation of damaged proteins. Recombinant human PCMT1 protien, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
|Synonyms||, Protein-L-isoaspartate O-methyltransferase L isoaspartyl protein carboxyl methyltransferase, L isoaspartyl/D aspartyl methyltransferase, PCMT 1, PIMT, Protein beta aspartate methyltransferase, Protein L isoaspartate (D aspartate) O methyltransferase, Protein L isoaspartyl/D aspartyl methyltransferase,|
|AA Sequence||MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMAWK SGGASHSELI HNLRKNGIIK TDKVFEVMLA TDRSHYAKCN PYMDSPQSIG FQATISAPHM HAYALELLFD QLHEGAKALD VGSGSGILTA CFARMVGCTG KVIGIDHIKE LVDDSINNVR KDDPTLLSSG RVQLVVGDGR MGYAEEAPYD AIHVGAAAPV VPQALIDQLK PGGRLILPVG PAGGNQMLEQ YDKLQDGSIK MKPLMGVIYV PLTDKEKQWS RWK|
|Molecular Weight||16.5 kDa (147aa) confirmed by MALDI-TOF|
|Form||Liquid, in 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 5mM DTT, 20% glycerol|
|Concentration||0.5 mg/ml (determined by Bradford assay)|
|Purity||> 95% by SDS-PAGE|
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Intended Use||For Research Use Only|
|References||Kagan RM., et al. (1997) Comp Biochem Physiol B Biochem Mol Biol. 117(3):379-85.|
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