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|Product Name||PGD, 1-483aa, Human|
|Amino Acid Sequence||MGSSHHHHHH SSGLVPRGSH MAQADIALIG LAVMGQNLIL NMNDHGFVVC AFNRTVSKVD DFLANEAKGT KVVGAQSLKE MVSKLKKPRR IILLVKAGQA VDDFIEKLVP LLDTGDIIID GGNSEYRDTT RRCRDLKAKG ILFVGSGVSG GEEGARYGPS LMPGGNKEAW PHIKTIFQGI AAKVGTGEPC CDWVGDEGAG HFVKMVHNGI EYGDMQLICE AYHLMKDVLG MAQDEMAQAF EDWNKTELDS FLIEITANIL KFQDTDGKHL LPKIRDSAGQ KGTGKWTAIS ALEYGVPVTL IGEAVFARCL SSLKDERIQA SKKLKGPQKF QFDGDKKSFL EDIRKALYAS KIISYAQGFM LLRQAATEFG WTLNYGGIAL MWRGGCIIRS VFLGKIKDAF DRNPELQNLL LDDFFKSAVE NCQDSWRRAV STGVQAGIPM PCFTTALSFY DGYRHEMLPA SLIQAQRDYF GAHTYELLAK PGQFIHTNWT GHGGTVSSSS YNA|
|Background||PGD (Phosphogluconate dehydrogenas), also known as 6PGD, is a 483 amino acid enzyme that is involved in the pentose phosphate shunt. Pentose is required for nucleic acid biosynthesis and the pentose phosphate cycle is a major source of NADPH. PGD deficiency increases the level of erythrocyte pyruvate kinase (PK) activity and reduces glutathione synthetase (GSH), resulting in hemolysis. Defects in PGD are generally asymptomatic and are inherited in an autosomal dominant fashion. Recombinant human PGD protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
|Concentration||0.5 mg/ml (determined by Bradford assay)|
|Form||Liquid, in Phosphate Buffered Saline (pH7.4) containing 20% glycerol, 0.1M NaCl|
|Intended Use||For Research Use Only|
|Molecular Weight||24.9 kDa (222aa), confirmed by MALDI-TOF.|
|NCBI Accession #||NP_002622|
|Purity||> 95% by SDS-PAGE|
|References||Le TD., et al. (2010) Proc Natl Acad Sci U S A. 107(7):3198-203.
Tagen M., et al. (2009) J Immunol. 183(10):6313-9.
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Synonyms||6PGD, Phosphogluconate dehydrogenase 0610042A05Rik, AU019875, C78335, 6 phosphogluconate dehydrogenase, decarboxylating, OTTMUSP00000011754, RP23-249G3.4,|
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