An ARP Customer Service Representative is happy to help!
|Product Name||Phospho-4EBP1 (T37/T46) TR-FRET Cellular Assay Kit|
Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-4EBP1 (T37/T46)
This assay kit measures intracellular levels of Phospho-4EBP1 (T37/T46) protein in cell lysates using a simple, rapid and sensitive immunoassay based on the homogeneous (no-wash) THUNDERTM TR-FRET technology. The kit is compatible with both adherent and suspension cells.
|Background||The Phospho-4EBP1 (T37/T46) assay kit is a homogeneous time-resolved Förster resonance energy transfer (TR-FRET) sandwich immunoassay (Figure 1). The THUNDERTM Cell Signaling assay workflow consists of 3 steps (Figure 2). Following cell treatment, cells are first lysed with the specific Lysis Buffer provided in the kit. Then Phospho-4EBP1 (T37/T46) in the cell lysates is detected with a pair of fluorophore-labeled antibodies in a simple "add-incubate- measure" format (single-step reagent addition; no wash steps). One antibody is labeled with a donor fluorophore (Europium chelate; Eu-Ab1) and the second with a far-red acceptor fluorophore (FR- Ab2). The binding of the two labeled antibodies to distinct epitopes on the target protein takes place in solution and brings the two dyes into close proximity. Excitation of the donor Europium chelate molecules with a flash lamp (320 or 340 nm) or a laser (337 nm) triggers a FRET from the donor to the acceptor molecules, which in turn emit a TR-FRET signal at 665 nm. Residual energy from the Eu chelate generates light at 615 nm. The signal at 665 nm is proportional to the concentration of Phospho-4EBP1 (T37/T46) in the cell lysate. Data can be expressed as either the signal at 665 nm or the 665 nm/615 nm ratio.|
|Sample Types||Cell lysates (adherent or suspension cells)|
|Intended Use||For research use only|
Components (100 Test):
Components (500 Test):
All Research Products are sold for laboratory RESEARCH USE ONLY and ARE NOT TO BE USED FOR HUMAN OR ANIMAL THERAPEUTIC OR DIAGNOSTIC APPLICATIONS. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. ARP American Research Products, Inc. does not submit its products for regulatory review by any government body or other organization, and we do not validate them for clinical, therapeutic or diagnostic use, or for safety and effectiveness. You are solely responsible for making sure that the way you use the products complies with applicable laws, regulations and governmental policies and for obtaining all necessary approvals, intellectual property rights, licenses and permissions that you may need related to your use. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process. ARP American Research Products, Inc. disclaims any and all representations or warranties of any kind whatsoever, express or implied, including without limitation any implied warranties of merchantability or fitness for a particular purpose, of non-infringement, or regarding results obtained through the use of any product, whether arising from a statute or otherwise in law or from a course of performance, dealing or usage of trade.