Pig 1,25-Dihydroxyvitamin D3 (1,25 DHVD3) ELISA Kit

Category: Assay Kits
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KTE80053
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Product Name Pig 1,25-Dihydroxyvitamin D3 (1,25 DHVD3) ELISA Kit
Description Pig 1,25-Dihydroxyvitamin D3 (1,25 DHVD3) ELISA Kit has high sensitivity and excellent specificity for detection of Pig 25-Dihydroxyvitamin. No significant cross-reactivity or interference between Pig 25-Dihydroxyvitamin and analogues was observed. 1,25-(OH)2D3, the active metabolite of vitamin D, regulates immune responses in addition to its role in calcium, phosphorus, and bone metabolism. Recent data suggests that 1,25-(OH)2D3 blocks dendritic cell maturation influencing the development of regulatory T cells. High dose 1,25-(OH)2D3 monotherapy is effective at delaying acute rejection. The vitamin D receptor (VDR), which functions as a transcription factor, forms a complex with Smad 3, a TGFb-1 signaling protein, in rat renal lysates for treated recipients. 1,25-(OH)2D3 significantly prolonged graft survival, limited the degree of intersitial fibrosis and glomerulosclerosis, decreased urinary protein and altered Smad and MMP expression in a rat renal model of CAN. In a retrospective clinical study, that cadaveric renal transplant recipients with renal insufficiency placed on calcitriol demonstrate improved renal function and improved graft survival. This Pig 1,25-Dihydroxyvitamin D3 (1,25 DHVD3) ELISA Kit employs a two-site sandwich ELISA to quantitate 25-Dihydroxyvitamin in samples. An antibody specific for 25-Dihydroxyvitamin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any25-Dihydroxyvitamin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for 25-Dihydroxyvitamin is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of 25-Dihydroxyvitamin bound in the initial step. The color development is stopped and the intensity of the color is measured.
Synonyms 25-Dihydroxyvitamin
Method Sandwich ELISA
Detection Range Please inquire
Sensitivity Please inquire
Reactivity Porcine
Sample Types Cell culture supernatants, Serum, Plasma, Other biological fluids
Storage 2-8°C
Supplier Abbkine

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