Pig Adrenomedullin (ADM) ELISA Kit

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KTE80055
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Product Name Pig Adrenomedullin (ADM) ELISA Kit
Description Pig Adrenomedullin (ADM) ELISA Kit has high sensitivity and excellent specificity for detection of Pig ADM. No significant cross-reactivity or interference between Pig ADM and analogues was observed. Adrenomedullin (ADM) is a peptide associated with pheochromocytoma. Adrenomedullin (AM) is a ubiquitously expressed peptide initially isolated from phaechromyctoma. Adrenomedullin, a hypotensive peptide found in human pheochromocytoma, consists of 52 amino acids, has 1 intramolecular disulfide bond, and shows a slight homology with the calcitonin gene-related peptide. It may function as a hormone in circulation control because it is found in blood in a considerable concentration. The precursor, called preproadrenomedullin, is 185 amino acids long. By RNA-blot analysis, human adrenomedullin mRNA was found to be highly expressed in several tissues. The human AM gene is localized to a single locus on Chromosome 11 with 4 exons and 3 introns. This Pig Adrenomedullin (ADM) ELISA Kit employs a two-site sandwich ELISA to quantitate ADM in samples. An antibody specific for ADM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADM is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADM bound in the initial step. The color development is stopped and the intensity of the color is measured.
Synonyms ADM, AM, preproadrenomedullin
Method Sandwich ELISA
Detection Range Please inquire
Sensitivity Please inquire
Reactivity Porcine
Sample Types Cell culture supernatants, Serum, Plasma, Other biological fluids
Gene Id 397195
Storage 2-8°C
Supplier Abbkine

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