| Product Name | Pig Protein S100-A6 (S100A6) ELISA Kit |
|---|---|
| Description | Pig Protein S100-A6 (S100A6) ELISA Kit has high sensitivity and excellent specificity for detection of Pig S100A6. No significant cross-reactivity or interference between Pig S100A6 and analogues was observed. Protein S100-A6 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-dependent insulin release, stimulation of prolactin secretion, and exocytosis. Chromosomal rearrangements and altered expression of this gene have been implicated in melanoma. This Pig Protein S100-A6 (S100A6) ELISA Kit employs a two-site sandwich ELISA to quantitate S100A6 in samples. An antibody specific for S100A6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyS100A6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S100A6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S100A6 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | S100A6, 2A9, 5B10, CABP, CACY, PRA, OTTHUMP00000015473, S100 calcium-binding protein A6, S100 calcium-binding protein A6 (calcyclin), calcyclin, prolactin receptor-associated protein |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Porcine |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 733608 |
| Storage | 2-8°C |
| Supplier | Abbkine |
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