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|Product Name||Pin 1 (Peptidyl-prolyl cis/trans isomerase) Human, Recombinant|
|Amino Acid Sequence||MADEEKLPPG WEKRMSRSSG RVYYFNHITN ASQWERPSGN SSSGGKNGQG EPARVRCSHL LVKHSQSRRP SSWRQEKITR TKEEALELIN GYIQKIKSGE EDFESLASQF SDCSSAKARG DLGAFSRGQM QKPFEDASFA LRTGEMSGPV FTDSGIHIIL RTE|
|Background||Human Pin 1 is a peptidyl-prolyl cis/trans isomerase (PPIase) that interacts with NIMA and essential for cell cycle regulation Pin1 is nuclear PPIase containing a WW protein interaction domain, and is structurally and functionally related to Ess1/Ptf1, an essential protein in budding yeast. PPIase activity is necessary for Ess1/Pin1 function in yeast. Pin1 is thus an essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Substrates of Pin1 include the mitotic regulators (Cdc25 phosphatase and NIMA ,PLK I, Wee, and Myt1 kinases), several transcription factors like beta-Catenin, c-Jun, and the tumor suppressor protein p53, and some specific proteins like the RNA Pol II, the cytoskeleton protein tau, and the G1/S protein Cyclin D1.|
|Concentration||1 mg/ml (determined by Bradford assay)|
|Endotoxin Level||< 1.0 EU per 1 microgram of protein (determined by LAL method)|
|Form||Liquid, in Phosphate-Buffered Saline (pH 7.4)|
|Intended Use||For Research Use Only|
|Molecular Weight||14 kDa (125 aa), confirmed by MALDI-TOF.|
|NCBI Accession #||NP_006212|
|Purity||95% by SDS-PAGE|
|References||Wulf GM., et al. (2002) J Biol. Chem. 277(50):47976-47979
Hamdane M., et al. (2002) J Mol Neurosci. 19(3); 275-287
Zheng H, et al. (2002) Nature. 419(6909) 853-857.
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Synonyms||PIN1, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, EC 188.8.131.52, Rotamase Pin1, PPIase Pin1, DOD, UBL5, PIN1, PPIase, EC 184.108.40.206, Rotamase Pin1, PPIase Pin1, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1|
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