POLR2F, 1-127aa, Human

Proteins, Recombinant
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Product Name POLR2F, 1-127aa, Human
Applications SDS-PAGE
Background POLR2F is the sixth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes, that is also shared by the other two DNA-directed RNA polymerases. In yeast, this polymerase subunit, in combination with at least two other subunits, forms a structure that stabilizes the transcribing polymerase on the DNA template. Recombinant human POLR2F protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Concentration 0.5 mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing
10% glycerol, 0.4M Urea
Species Human
Host E.coli
Intended Use For Research Use Only
Molecular Weight 27.7 kDa (247 aa)
NCBI Accession # NP_068809
Purity > 95% by SDS-PAGE
References Kayukawa K., Makino Y., et al. (1999) Gene 234:139-147
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Synonyms DNA-directed RNA polymerases I, II, and III subunit RPABC2, Polymerase (RNA) II (DNA directed) polypeptide F, HRBP14.4, POLRF, RPABC2, RPB14.4, RPB6
Tag His-tag

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