Product Name | PPP3CA, 1-511aa, Human |
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Description | PPP3CA, also known as Calcineurin A, is a major soluble calmodulin binding protein in the brain, a Ca2+/calmodulin dependent serine/threonine protein phosphatase, with a relatively narrow substrate specificity. It activates the T cells of the immune system and can be blocked by drugs. PPP3CA activates NFATc, a transcription factor by dephosphorylating it. The activated NFATc is then translocated into the nucleus, where it upregulates the expression of interleukin 2. Recombinant human PPP3CA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
Synonyms | Serine/threonine-protein phosphatase 2B catalytic subunit alpha, CALN, CALNA, CALNA1, CCN1, CNA1, PPP2B |
Host | E.coli |
Molecular Weight | 60.6 kDa (559aa) |
Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH TGSMSEPKAI DPKLSTTDRV VKAVPFPPSH RLTAKEVFDN DGKPRVDILK AHLMKEGRLE ESVALRIITE GASILRQEKN LLDIDAPVTV CGDIHGQFFD LMKLFEVGGS PANTRYLFLG DYVDRGYFSI ECVLYLWALK ILYPKTLFLL RGNHECRHLT EYFTFKQECK IKYSERVYDA CMDAFDCLPL AALMNQQFLC VHGGLSPEIN TLDDIRKLDR FKEPPAYGPM CDILWSDPLE DFGNEKTQEH FTHNTVRGCS YFYSYPAVCE FLQHNNLLSI LRAHEAQDAG YRMYRKSQTT GFPSLITIFS APNYLDVYNN KAAVLKYENN VMNIRQFNCS PHPYWLPNFM DVFTWSLPFV GEKVTEMLVN VLNICSDDEL GSEEDGFDGA TAAARKEVIR NKIRAIGKMA RVFSVLREES ESVLTLKGLT PTGMLPSGVL SGGKQTLQSA IKGFSPQHKI TSFEEAKGLD RINERMPPRR DAMPSDANLN SINKALTSET NGTDSNGSNS SNIQ |
Tag | His-tag |
Reactivity | Human |
Applications | SDS-PAGE |
Form | Liquid, in 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 5mM DTT, 1mM EDTA, 20% glycerol |
Concentration | 0.25 mg/ml (determined by Bradford assay) |
Purity | > 85% by SDS-PAGE |
Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
References |
Ostenfeld MS., et al. (2010) Oncogene. 29(7):1073-84. Rodriguez A., et al. (2009) Mol Cell. 33(5):616-26. |
Supplier | ARP |
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