AAV-1 Titration ELISA

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PRAAV1
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Product Name AAV-1 Titration ELISA
Description Enzyme Immunoassay for the Quantitative Determination of AAV Serotype 1 Particles in Cell Culture Supernatants and Purified Virus Preparations.
Background Adeno-associated virus (AAV) is a ssDNA virus that is a topic of intense study in gene therapy. The different serotypes transduce a wide variety of dividing and non-dividing cells showing long-term gene expression. AAV 1 has been used in several studies showing superior infection of muscle, liver and vascular endothelium when compared to other AAV serotypes. Until now no serious vector-related adverse effects have been observed. Methods for the characterisation of AAV preparations currently include titration ELISA, real-time PCR, DNA dot blot, determination of transducing units, infectious center assay, SDS- PAGE or electron microscopy.

Immunotitration by ARP's AAV 1 Titration ELISA offers a fast, sensitive and reproducible method for titration of intact AAV 1 wt virions, AAV 1 recombinant virions or assembled and intact empty AAV 1 capsids.
Principle The assay is based on the sandwich ELISA technique. A monoclonal antibody specific for a conformational epitope on assembled AAV 1 capsids is coated onto microtiter strips and is used to capture AAV 1 particles from the specimen. Captured AAV 1 particles are detected in two steps. First a biotin conjugated monoclonal antibody to AAV 1 is bound to the immune complex. In the second step streptavidin peroxidase conjugate reacts with the biotin molecules. Addition of substrate solution results in a color reaction which is proportional to the amount of specifically bound viral particles. The absorbance is measured photometrically at 450 nm.

The kit control provided contains an AAV 1 particle preparation of intact capsids. It shows a typical titration curve when used in dilutions of steps of two (Fig. 1). It allows quantitative determination of samples of an unknown particle titer (immunological titer) and the calibration of an inhouse AAV 1 preparation (e.g. infectious titer, transducing units, DNA dot blot titer, Table 1).
Storage 2-8C
Intended Use For Research Use Only

All Research Products are sold for laboratory RESEARCH USE ONLY and ARE NOT TO BE USED FOR HUMAN OR ANIMAL THERAPEUTIC OR DIAGNOSTIC APPLICATIONS. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. ARP American Research Products, Inc. does not submit its products for regulatory review by any government body or other organization, and we do not validate them for clinical, therapeutic or diagnostic use, or for safety and effectiveness. You are solely responsible for making sure that the way you use the products complies with applicable laws, regulations and governmental policies and for obtaining all necessary approvals, intellectual property rights, licenses and permissions that you may need related to your use. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process. ARP American Research Products, Inc. disclaims any and all representations or warranties of any kind whatsoever, express or implied, including without limitation any implied warranties of merchantability or fitness for a particular purpose, of non-infringement, or regarding results obtained through the use of any product, whether arising from a statute or otherwise in law or from a course of performance, dealing or usage of trade.

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