Anti-Adeno-associated virus (AAV), intact particles Biotin

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03-61555
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Antibodies, Monoclonal Antibodies, Anti-AAV Products
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Product Name Anti-Adeno-associated virus (AAV), intact particles Biotin
Description For characterization of different stages of infection and very useful for the analysis of the AAV assembly process. The antibody cannot be used for immunoblotting. Specifically detects intact virus particles, both empty and full capsids. With AAV-2 and AAV-3 A20 recognizes a conformational epitope of assembled capsids, not present in denatured and native unassembled capsid proteins.
Background Adeno-associated virus (AAV) is a small virus which infects humans and some other primate species. AAV is not currently known to cause disease and consequently the virus causes a very mild immune response. Gene therapy vectors using AAV can infect both dividing and quiescent cells and persist in an extrachromosomal state without integrating into the genome of the host cell. These features make AAV a very attractive candidate for creating viral vectors for gene therapy, and for the creation of isogenic human disease models
Clone A20
Conjugate Biotin
Immunogen Adeno-associated virus capsid proteins and virus particles
Specificity A20 specifically reacts with intact adeno-associated virus particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids, not present in denatured capsid proteins and native but unassembled capsid proteins. Epitope mapping experiments (Wobus et al., see below) identified four immunoreactive (discontinous) regions. The major reaction was attrib- uted to sequence aa 369 to aa378 of AAV-2 capsids. The antibody is also useful for neutralizing experiments (cf. Moskalenko et.al)
Form Purified (Protein A), in PBS containing 1% BSA and 0.1% sodium-azide
Reactivity AAV-2 found in human & monkey. In ELISA also applicable to AAV-3
Applications ELISA, IF, IHC
Working Dilutions 1:20 for immunofluorescence microscopy and ELISA; 1:5 for immunoprecipitation
Storage 2-8C for immediate use, or at -20C (aliquot)
Intended Use For Research Use Only
Isotype IgG3
Incubation Time ELISA: 1 hour at 37C

Immunohistochemistry and Immunofluorescence microscopy: Overnight at 2-8C

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