Anti-HPRT (clone AT2G8)

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01-A0204
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Antibodies, Monoclonal Antibodies
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Product Name Anti-HPRT (clone AT2G8)
Description HPRT is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. HPRT, which acts as a catalyst in the reaction between guanine and phosphoribosyl pyrophosphate to form GMP, functions primarily to salvage purines from degraded DNA to renewed purine synthesis.
Synonyms HGPRT, HGPRTase, HPRT, Hypoxanthine-guanine phosphoribosyltransferase HPRT 1, HPRT1, Hypoxanthine guanine phosphoribosyltransferase, Hypoxanthine phosphoribosyltransferase 1 (Lesch Nyhan syndrome), Hypoxanthine phosphoribosyltransferase 1.
Host Mouse
Clone AT2G8. Anti-human HPRT mAb, clone AT2G8, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human HPRT protein.
Immunogen Recombinant human HPRT (1-218aa) purified from E. coli
Form Purified, in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide
Reactivity Human
Applications Dot blot, ELISA, WB
Concentration 0.1 mg/ml
Working Dilutions Optimal dilution should be performed by user.
Species Mouse
Isotype IgG1, K
Interest Fields Signal Transduction
NCBI Number NP_000185
Storage Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.
References Sculley DG, et al. (1993). Hum. Genet. 90 (3): 195-207. Stout JT, Caskey CT (1986). Annu. Rev. Genet. 19: 127-48. Davidson BL, et al. (1991). Am. J. Hum. Genet. 48 (5): 951-8.

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