Cow 14-3-3 protein theta (YWHAQ) ELISA Kit

Catalog
KTE10001
$0.00
-
+
Assay Kits, ELISA, Cow ELISA, Abbkine
(Ships in 5-10 business days)
Questions? Contact Us
More Information
Product Name Cow 14-3-3 protein theta (YWHAQ) ELISA Kit
Species Cow
Description This Bovine 14-3-3 protein theta (YWHAQ) ELISA Kit employs a two-site sandwich ELISA to quantitate YWHAQ in samples. An antibody specific for YWHAQ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyYWHAQ present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for YWHAQ is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of YWHAQ bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background YWHAq product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease.The deduced 245-amino acid protein has a calculated molecular mass of 28 kD and shares 72% amino acid identity with bovine brain 14-3-3-eta. Northern blot analysis of Jurkat and HeLa cells showed strong bands at 1.9 and 2.1 kb, and a weaker band at 1.7 kb.
Synonyms YWHAQ, 14-3-3, 1C5, HS1, 14-3-3 protein T-cell, 14-3-3 protein tau, 14-3-3 protein theta, OTTHUMP00000165887, protein tau, tyrosine 3/tryptophan 5 -monooxygenase activation protein, theta polypeptide
Method Sandwich ELISA
Sample Types Cell culture supernatants, Serum, Plasma, Other biological fluids
Gene ID 768311
Intended Use For Research Use Only
Uniprot Accession ID Q3SZI4

All Research Products are sold for laboratory RESEARCH USE ONLY and ARE NOT TO BE USED FOR HUMAN OR ANIMAL THERAPEUTIC OR DIAGNOSTIC APPLICATIONS. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. ARP American Research Products, Inc. does not submit its products for regulatory review by any government body or other organization, and we do not validate them for clinical, therapeutic or diagnostic use, or for safety and effectiveness. You are solely responsible for making sure that the way you use the products complies with applicable laws, regulations and governmental policies and for obtaining all necessary approvals, intellectual property rights, licenses and permissions that you may need related to your use. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process. ARP American Research Products, Inc. disclaims any and all representations or warranties of any kind whatsoever, express or implied, including without limitation any implied warranties of merchantability or fitness for a particular purpose, of non-infringement, or regarding results obtained through the use of any product, whether arising from a statute or otherwise in law or from a course of performance, dealing or usage of trade.

Customers Also Viewed
  1. Alpha-1 Antichymotrypsin, Human
    Alpha-1 Antichymotrypsin,...
    12-7602
    An acute-phase plasm...
  2. Western blot
All lanes: Vinculin antibody at 2ug/ml+293T whole cell lysate
Secondary
Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 124,117,25 kDa
Observed band size: 125 kDa
    Anti-VCL Polyclonal Antib...
    CSB-PA13779A0Rb
    ELISA, WB
    Human

Hi, have questions?

An ARP Customer Service Representative is happy to help!

or Call (800) 832-2611