Cow 14-3-3 protein theta (YWHAQ) ELISA Kit

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AE10851BO
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Assay Kits, ELISA, Cow ELISA, Abebio
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Product Name Cow 14-3-3 protein theta (YWHAQ) ELISA Kit
Species Bovine (Bos taurus, Cattle)
Description This assay has high sensitivity and excellent specificity for detection of Bovine YWHAQ. No significant cross-reactivity or interference between Bovine YWHAQ and analogues was observed.
Background YWHAq product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease.The deduced 245-amino acid protein has a calculated molecular mass of 28 kD and shares 72% amino acid identity with bovine brain 14-3-3-eta. Northern blot analysis of Jurkat and HeLa cells showed strong bands at 1.9 and 2.1 kb, and a weaker band at 1.7 kb.
Abbreviation YWHAQ
Synonyms 14-3-3, 1C5, HS1, 14-3-3 protein T-cell|14-3-3 protein tau|14-3-3 protein theta|OTTHUMP00000165887|protein tau|tyrosine 3/tryptophan 5 -monooxygenase activation protein, theta polypeptide
Method Sandwich ELISA
Detection Range 0.156-10 ng/mL
Sensitivity 0.053 ng/mL
Sample Types Serum, Plasma, Other biological fluids.
Uniprot No. Q3SZI4
Principle of the Assay This assay employs a two-site sandwich ELISA to quantitate YWHAQ in samples. An antibody specific for YWHAQ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyYWHAQ present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for YWHAQ is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of YWHAQ bound in the initial step. The color development is stopped and the intensity of the color is measured.

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