Cow 28S ribosomal protein S21, mitochondrial (MRPS21) ELISA Kit

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KTE10271
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Assay Kits, ELISA, Cow ELISA, Abbkine
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Product Name Cow 28S ribosomal protein S21, mitochondrial (MRPS21) ELISA Kit
Species Cow
Description This Bovine 28S ribosomal protein S21, mitochondrial (MRPS21) ELISA Kit employs a two-site sandwich ELISA to quantitate MRPS21 in samples. An antibody specific for MRPS21 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMRPS21 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MRPS21 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MRPS21 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. MRPS21 encodes a 28S subunit protein that belongs to the ribosomal protein S21P family. Pseudogenes corresponding to MRPS21 are found on chromosomes 1p, 1q, 9p, 10p, 10q, 16q, and 17q. Available sequence data analyses identified splice variants that differ in the 5' UTR; both transcripts encode the same protein.
Synonyms MRPS21, MDS016, MRP-S21, RPMS21, mitochondrial 28S ribosomal protein S21
Method Sandwich ELISA
Sample Types Cell culture supernatants, Serum, Plasma, Other biological fluids
Gene ID 614343
Intended Use For Research Use Only
Uniprot Accession ID P82920

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