Cow 28S ribosomal protein S22, mitochondrial (MRPS22) ELISA Kit

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KTE10270
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Assay Kits, ELISA, Cow ELISA, Abbkine
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Product Name Cow 28S ribosomal protein S22, mitochondrial (MRPS22) ELISA Kit
Species Cow
Description This Bovine 28S ribosomal protein S22, mitochondrial (MRPS22) ELISA Kit employs a two-site sandwich ELISA to quantitate MRPS22 in samples. An antibody specific for MRPS22 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMRPS22 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MRPS22 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MRPS22 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. MRPS22 encodes a 28S subunit protein that does not seem to have a counterpart in prokaryotic and fungal-mitochondrial ribosomes. MRPS22 lies telomeric of and is transcribed in the opposite direction from the forkhead box L2 gene. A pseudogene corresponding to MRPS22 is found on chromosome Xq.
Synonyms MRPS22, C3orf5, COXPD5, GIBT, GK002, MRP-S22, RPMS22,
Method Sandwich ELISA
Sample Types Cell culture supernatants, Serum, Plasma, Other biological fluids
Gene ID 532044
Uniprot Accession ID P82649
Intended Use For Research Use Only

All Research Products are sold for laboratory RESEARCH USE ONLY and ARE NOT TO BE USED FOR HUMAN OR ANIMAL THERAPEUTIC OR DIAGNOSTIC APPLICATIONS. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. ARP American Research Products, Inc. does not submit its products for regulatory review by any government body or other organization, and we do not validate them for clinical, therapeutic or diagnostic use, or for safety and effectiveness. You are solely responsible for making sure that the way you use the products complies with applicable laws, regulations and governmental policies and for obtaining all necessary approvals, intellectual property rights, licenses and permissions that you may need related to your use. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process. ARP American Research Products, Inc. disclaims any and all representations or warranties of any kind whatsoever, express or implied, including without limitation any implied warranties of merchantability or fitness for a particular purpose, of non-infringement, or regarding results obtained through the use of any product, whether arising from a statute or otherwise in law or from a course of performance, dealing or usage of trade.

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