Cow 28S ribosomal protein S25, mitochondrial (MRPS25) ELISA Kit

Catalog
KTE10267
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Assay Kits, ELISA, Cow ELISA, Abbkine
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Product Name Cow 28S ribosomal protein S25, mitochondrial (MRPS25) ELISA Kit
Species Cow
Description This Bovine 28S ribosomal protein S25, mitochondrial (MRPS25) ELISA Kit employs a two-site sandwich ELISA to quantitate MRPS25 in samples. An antibody specific for MRPS25 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMRPS25 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MRPS25 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MRPS25 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. MRPS25 encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4. Alternative splicing results in multiple transcript variants.
Synonyms MRPS25, DKFZp313H0817, FLJ00023, MRP-S25, RPMS25, mitochondrial 28S ribosomal protein S25
Method Sandwich ELISA
Sample Types Cell culture supernatants, Serum, Plasma, Other biological fluids
Gene ID 533011
Uniprot Accession ID P82669
Intended Use For Research Use Only

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