Cow 7-dehydrocholesterol reductase (DHCR7) ELISA Kit

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AE47348BO
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Assay Kits, ELISA, Cow ELISA, Abebio
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Product Name Cow 7-dehydrocholesterol reductase (DHCR7) ELISA Kit
Species Bovine (Bos taurus, Cattle)
Description This assay has high sensitivity and excellent specificity for detection of Bovine DHCR7. No significant cross-reactivity or interference between Bovine DHCR7 and analogues was observed.
Abbreviation DHCR7
Synonyms SLOS, delta-7-dehydrocholesterol reductase|sterol delta-7-reductase
Method Sandwich ELISA
Detection Range Request Information
Sensitivity Request Information
Sample Types Serum, Plasma, Other biological fluids.
Uniprot No. Q5E9J5
Principle of the Assay This assay employs a two-site sandwich ELISA to quantitate DHCR7 in samples. An antibody specific for DHCR7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDHCR7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DHCR7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DHCR7 bound in the initial step. The color development is stopped and the intensity of the color is measured.

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