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|Product Name||Cow Acetylcholine (ACH) ELISA Kit|
|Species||Bovine (Bos taurus, Cattle)|
|Description||This assay has high sensitivity and excellent specificity for detection of Bovine ACH. No significant cross-reactivity or interference between Bovine ACH and analogues was observed.|
|Background||Acetylcholine (Ach) is produced by the synthetic enzyme choline acetyltransferase which uses acetyl coenzyme A and choline as substrates for the formation of acetylcholine.Dietary choline and phosphatidylcholine serve as the sources of free choline for acetylcholine synthesis. Upon release, acetylcholine is metabolized into choline and acetate by acetylcholinesterase, and other nonspecific esterases. Acetylcholine release can be excitatory or inhibitory depending on the type of tissue and the nature of the receptor with which it interacts. Cholinergic receptors can be divided into two types, muscarinic and nicotinic, based on the pharmacological action of various agonists and antagonists. Muscarinic receptors originally were distinguished from nicotinic receptors by the selectivity of the agonists muscarine and nicotine respectively.|
|Detection Range||12.35-1000 pg/mL|
|Sample Types||Serum, Plasma, Other biological fluids.|
|Principle of the Assay||This assay employs a two-site sandwich ELISA to quantitate ACH in samples. An antibody specific for ACH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACH bound in the initial step. The color development is stopped and the intensity of the color is measured.|
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