Cow Acetylcholinesterase (ACHE) ELISA Kit

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AE23411BO
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Assay Kits, ELISA, Cow ELISA, Abebio
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Product Name Cow Acetylcholinesterase (ACHE) ELISA Kit
Species Bovine (Bos taurus, Cattle)
Description This assay has high sensitivity and excellent specificity for detection of Bovine ACHE. No significant cross-reactivity or interference between Bovine ACHE and analogues was observed.
Background Acetylcholinesterase is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group. It is mainly found at neuromuscular junctions and cholinergic synapses in the central nervous system, where its activity serves to terminate synaptic transmission.
AChE has a very high catalytic activity mdash; each molecule of AChE degrades about 5000 molecules of acetylcholine per second. The choline produced by the action of AChE is recycled mdash; it is transported, through reuptake, back into nerve terminals where it is used to synthesize new acetylcholine molecules.Acetylcholinesterase is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen.
Abbreviation ACHE
Synonyms ARACHE, N-ACHE, YT, OTTHUMP00000211347|OTTHUMP00000211349|OTTHUMP00000211356|acetylcholinesterase|apoptosis-related acetylcholinesterase
Method Sandwich ELISA
Detection Range 0.94-60 U/mL
Sensitivity 0.24 U/mL
Sample Types Serum, Plasma, Other biological fluids.
Uniprot No. P23795
Principle of the Assay This assay employs a two-site sandwich ELISA to quantitate ACHE in samples. An antibody specific for ACHE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACHE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACHE is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACHE bound in the initial step. The color development is stopped and the intensity of the color is measured.

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