Cow Adiponectin (ADP) ELISA Kit

Catalog
AE23983BO
$0.00
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Assay Kits, ELISA, Cow ELISA, Abebio
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Product Name Cow Adiponectin (ADP) ELISA Kit
Species Bovine (Bos taurus, Cattle)
Description This assay has high sensitivity and excellent specificity for detection of Bovine ADP. No significant cross-reactivity or interference between Bovine ADP and analogues was observed.
Background Adiponectin, alternatively named Adipocyte Complement-Related Protein of 30 kDa, shares structural similarity with complement factor C1q and is a member of the family of defense collagens. It is secreted exclusively by differentiated adipocytes and circulates at high concentrations. Adiponectin has a modular structure comprising an N-terminal collagenous domain with multiple collagen triple helix repeats, followed by a C-terminal C1q-like globular domain. The globular domain has similar folding topology with tumor necrosis factor-_ and assembles into homotrimers. Higher order oligomeric adiponectins are also formed via interactions between the collagenous stalk. A truncated form of Adiponectin containing only the globular domain can be generated by proteolytic cleavage.
Abbreviation ADP
Synonyms ACDC, ACRP30, ADIPQTL1, ADPN, APM-1, APM1, GBP28, adiponectin, OTTHUMP00000210047|OTTHUMP00000210048|adipocyte, C1Q and collagen domain containing|adipocyte, C1Q and collagen domain-containing|adipo
Method Sandwich ELISA
Detection Range 0.781-50 ng/mL
Sensitivity 0.35 ng/mL
Sample Types Serum, Plasma, Other biological fluids.
Uniprot No. Q3Y5Z3
Principle of the Assay This assay employs a two-site sandwich ELISA to quantitate ADP in samples. An antibody specific for ADP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADP bound in the initial step. The color development is stopped and the intensity of the color is measured.

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