Cow Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A (MGAT4A) ELISA Kit

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KTE10298
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Assay Kits, ELISA, Cow ELISA, Abbkine
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Product Name Cow Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A (MGAT4A) ELISA Kit
Species Cow
Description This Bovine Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A (MGAT4A) ELISA Kit employs a two-site sandwich ELISA to quantitate MGAT4A in samples. An antibody specific for MGAT4A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMGAT4A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MGAT4A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MGAT4A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A (MGAT4A) gene encodes a key glycosyltransferase that regulates the formation of tri- and multiantennary branching structures in the Golgi apparatus. The encoded protein, in addition to the related isoenzyme B, catalyzes the transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc in a beta-1,4 linkage to the Man-alpha-1,3-Man-beta-1,4-GlcNAc arm of R-Man-alpha-1,6(GlcNAc-beta-1,2-Man-alpha-1,3)Man-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1-Asn. The encoded protein may play a role in regulating the availability of serum glycoproteins, oncogenesis, and differentiation. This protein acts as an enzyme. It is known to catalyze the following reaction :
UDP-N-acetyl-D-glucosamine + 3-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 3-(2,4-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.
Synonyms MGAT4A, GNT-IV, GNT-IVA, N-acetylglucosaminyltransferase IVa, OTTHUMP00000161122, OTTHUMP00000203150, UDP-GlcNAc:a-1,3-D-mannoside b-1,4-acetylglucosaminyltransferase IV, UDP-N-acetylglucosamine:alpha1,3-d-mann
Method Sandwich ELISA
Sample Types Cell culture supernatants, Serum, Plasma, Other biological fluids
Gene ID 282276
Intended Use For Research Use Only
Uniprot Accession ID O77836

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