Cow Alpha-2-macroglobulin (A2M) ELISA Kit

Assay Kits, ELISA, Cow ELISA, Abebio
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Product Name Cow Alpha-2-macroglobulin (A2M) ELISA Kit
Species Bovine (Bos taurus, Cattle)
Description This assay has high sensitivity and excellent specificity for detection of Bovine A2M. No significant cross-reactivity or interference between Bovine A2M and analogues was observed.
Background Alpha2-macroglobulin is a major serum protein with diverse functions, including inhibition of protease activity and binding of growth factors, cytokines, and disease factors. It is also a panproteinase inhibitor that is found immunohistochemically in neuritic plaques.
Alpha-2-macroglobulin is able to inactivate an enormous variety of proteinases (including serine-, cysteine-, aspartic- and metalloproteinases). Alpha-2-macroglobulin has in its structure a 35 aminoacid "bait" region. Proteinases binding and cleaving the bait region become bound to alpha2M. The proteinase-_2M complex is recognised by macrophage receptors and cleared from the system
Abbreviation A2M
Synonyms CPAMD5, DKFZp779B086, FWP007, S863-7,
Method Sandwich ELISA
Detection Range 0.312- 20 _g/mL
Sensitivity 0.078 _g/mL
Sample Types Serum, Plasma, Other biological fluids.
Uniprot No. Q7SIH1
Principle of the Assay This assay employs a two-site sandwich ELISA to quantitate A2M in samples. An antibody specific for A2M has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyA2M present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for A2M is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of A2M bound in the initial step. The color development is stopped and the intensity of the color is measured.

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