Cow Alpha-fetoprotein (AFP) ELISA Kit

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AE24192BO
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Assay Kits, ELISA, Cow ELISA, Abebio
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Product Name Cow Alpha-fetoprotein (AFP) ELISA Kit
Species Bovine (Bos taurus, Cattle)
Description This assay has high sensitivity and excellent specificity for detection of Bovine AFP. No significant cross-reactivity or interference between Bovine AFP and analogues was observed.
Background AFP is a glycoprotein with a molecular weight of between 65,000 and 70,000 daltons including 4% of carbohydrate . During fetal development, AFP maintains high levels in the serum and drops to very low levels throughout the remainder of life.
AFP is elevated in the malignant diseases of hepatocellular, testicular nonseminomatous origin, and occasionally of other entodermal origin. AFP may be slightly elevated or persisted in the patients with large hepatic metastases or viral hepatitis. AFP measurement is widely accepted as tumor marker and for monitoring the therapeutic effectiveness of hepatocellular cancer and nonseminomatous testicular cancer .
Abbreviation AFP
Synonyms FETA, HPAFP, OTTHUMP00000160480|alpha-1-fetoprotein|alpha-fetoglobulin
Method Sandwich ELISA
Detection Range 0.312-20 ng/mL
Sensitivity 0.118 ng/mL
Sample Types Serum, Plasma, Other biological fluids.
Uniprot No. Q3SZ57
Principle of the Assay This assay employs a two-site sandwich ELISA to quantitate AFP in samples. An antibody specific for AFP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAFP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AFP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AFP bound in the initial step. The color development is stopped and the intensity of the color is measured.

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