Cow Anti-Mullerian hormone (AMH) ELISA Kit

Assay Kits, ELISA, Cow ELISA, Abebio
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Product Name Cow Anti-Mullerian hormone (AMH) ELISA Kit
Species Bovine (Bos taurus, Cattle)
Description This assay has high sensitivity and excellent specificity for detection of Bovine AMH. No significant cross-reactivity or interference between Bovine AMH and analogues was observed.
Background Anti-MÙllerian hormone (AMH) is a dimeric glycoprotein that inhibits the development of the MÙllerian ducts in a male embryo. It is named after Johannes Peter MÙller. AMH is a protein hormone structurally related to inhibin and activin, and a member of the transforming growth factor-_ (TGF-_) family. It is present in fish, reptiles, birds, marsupials, and placental mammals.In mammals AMH is secreted by Sertoli cells of the testes during embryogenesis of the fetal male and prevents the development of the mullerian ducts into the uterus and other mullerian structures. The effect is ipsilateral, that is each testis suppresses MÙllerian development only on its own side. In humans this action takes place by 8 weeks gestation. In female embryogenesis the absence of AMH allows for the development of upper vagina, uterus and cervix, and oviducts.
Abbreviation AMH
Synonyms MIF, MIS, Mullerian inhibiting factor|Mullerian inhibiting substance
Method Sandwich ELISA
Detection Range 493.83-40000 pg/mL
Sensitivity 177.4 pg/mL
Sample Types Serum, Plasma, Other biological fluids.
Uniprot No. P03972
Principle of the Assay This assay employs a two-site sandwich ELISA to quantitate AMH in samples. An antibody specific for AMH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAMH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AMH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AMH bound in the initial step. The color development is stopped and the intensity of the color is measured.

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