Cow Apoptosis-associated speck-like protein containing a CARD (PYCARD) ELISA Kit

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AE24933BO
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Assay Kits, ELISA, Cow ELISA, Abebio
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Product Name Cow Apoptosis-associated speck-like protein containing a CARD (PYCARD) ELISA Kit
Species Bovine (Bos taurus, Cattle)
Description This assay has high sensitivity and excellent specificity for detection of Bovine PYCARD. No significant cross-reactivity or interference between Bovine PYCARD and analogues was observed.
Background PYCARD encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery.The deduced 195-amino acid protein contains an N-terminal pyrin-like domain (PYD) and an 87-residue C-terminal CARD. Fluorescence microscopy demonstrated a ring-like expression in some transfected cells.
Abbreviation PYCARD
Synonyms ASC, CARD5, MGC10332, TMS, TMS-1, TMS1, apoptosis-associated speck-like protein containing a CARD|caspase recruitment domain protein 5|target of methylation-induced silencing-1
Method Sandwich ELISA
Detection Range Request Information
Sensitivity Request Information
Sample Types Serum, Plasma, Other biological fluids.
Uniprot No. Q8HXK9
Principle of the Assay This assay employs a two-site sandwich ELISA to quantitate PYCARD in samples. An antibody specific for PYCARD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPYCARD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PYCARD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PYCARD bound in the initial step. The color development is stopped and the intensity of the color is measured.

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