Cow CAMP-dependent protein kinase catalytic subunit beta (PRKACB) ELISA Kit

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AE26005BO
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Assay Kits, ELISA, Cow ELISA, Abebio
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Product Name Cow CAMP-dependent protein kinase catalytic subunit beta (PRKACB) ELISA Kit
Species Bovine (Bos taurus, Cattle)
Description This assay has high sensitivity and excellent specificity for detection of Bovine PRKACB. No significant cross-reactivity or interference between Bovine PRKACB and analogues was observed.
Background cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the AMP-activated protein kinase (AMPK), which transduces the signal through phosphorylation of different target proteins. The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of AMPK have been identified in humans.
PRKACB is a member of the Ser/Thr protein kinase family and is a catalytic subunit of AMPK. Three alternatively spliced transcript variants encoding distinct isoforms have been observed.
Abbreviation PRKACB
Synonyms RP11-82H13.1, DKFZp781I2452, MGC41879, MGC9320, PKACB, PKA C-beta|cAMP-dependent protein kinase catalytic beta subunit isoform 4ab|cAMP-dependent protein kinase catalytic subunit beta|protein kinase
Method Sandwich ELISA
Detection Range Request Information
Sensitivity Request Information
Sample Types Serum, Plasma, Other biological fluids.
Uniprot No. P05131
Principle of the Assay This assay employs a two-site sandwich ELISA to quantitate PRKACB in samples. An antibody specific for PRKACB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRKACB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRKACB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKACB bound in the initial step. The color development is stopped and the intensity of the color is measured.

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