Cow CAMP-dependent protein kinase type II-alpha regulatory subunit (PRKAR2A) ELISA Kit

Catalog
KTE10181
$0.00
-
+
Assay Kits, ELISA, Cow ELISA, Abbkine
(Ships in 5-10 business days)
Questions? Contact Us
More Information
Product Name Cow CAMP-dependent protein kinase type II-alpha regulatory subunit (PRKAR2A) ELISA Kit
Species Cow
Description This Bovine CAMP-dependent protein kinase type II-alpha regulatory subunit (PRKAR2A) ELISA Kit employs a two-site sandwich ELISA to quantitate PRKAR2A in samples. An antibody specific for PRKAR2A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRKAR2A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRKAR2A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKAR2A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background The inactive holoenzyme of PKA is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of PKA have been identified in humans.
PRKAR2a is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of PKA. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER).
Synonyms PRKAR2A, MGC3606, PKR2, PRKAR2, OTTHUMP00000210266, cAMP-dependent protein kinase regulatory subunit RII alpha, cAMP-dependent protein kinase, regulatory subunit alpha 2, protein kinase A, RII-alpha subunit
Method Sandwich ELISA
Sample Types Cell culture supernatants, Serum, Plasma, Other biological fluids
Gene ID 100139910
Intended Use For Research Use Only
Uniprot Accession ID P00515

All Research Products are sold for laboratory RESEARCH USE ONLY and ARE NOT TO BE USED FOR HUMAN OR ANIMAL THERAPEUTIC OR DIAGNOSTIC APPLICATIONS. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. ARP American Research Products, Inc. does not submit its products for regulatory review by any government body or other organization, and we do not validate them for clinical, therapeutic or diagnostic use, or for safety and effectiveness. You are solely responsible for making sure that the way you use the products complies with applicable laws, regulations and governmental policies and for obtaining all necessary approvals, intellectual property rights, licenses and permissions that you may need related to your use. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process. ARP American Research Products, Inc. disclaims any and all representations or warranties of any kind whatsoever, express or implied, including without limitation any implied warranties of merchantability or fitness for a particular purpose, of non-infringement, or regarding results obtained through the use of any product, whether arising from a statute or otherwise in law or from a course of performance, dealing or usage of trade.

Customers Also Viewed
  1. Anti-p21WAF1 (Tumor Suppressor Protein), Monoclonal Antibody
    Anti-p21WAF1 (Tumor Suppr...
    24-1026-MSM4
    This monoclonal anti...
    FC, IF, IHC
    Chimpanzee, Human, M...
  2. Pig IL-1_ (Interleukin 1 Beta) ELISA Kit
    Pig IL-1_ (Interleukin 1 ...
    E-EL-P0002
    This ELISA kit uses ...

Hi, have questions?

An ARP Customer Service Representative is happy to help!

or Call (800) 832-2611